Promoter Methylation of Two <i>HOXA9</i> and <i>NISCH</i> Genes in Opium Users

Background: Opiate abuse has been critically increased in the world, especially Iran. Owing to association of opiate use with multiple human cancers and neurological disorders, seeking for genetic epigenetic effects opium can pave way early diagnosis major health defects addicted users. Accordingly, present study aimed determine methylation status promoter two genes, which are actively involved neurodevelopment cancer evolution. Methods: DNA was isolated from peripheral blood 28 abusers 19 healthy controls then subjected sonication. Sonicated DNAs undergone methylated immunoprecipitation-real time polymerase chain reaction (MeDIP-Real Time PCR) using specific primer pairs designed HOXA9 NISCH genes. Obtained data were analyzed SPSS software. Findings: genes found be significantly users compared (P&lt;0.001) associated mean age regarding gene (P=0.002). Neither amount nor duration or route Conclusion: Hypermethylation as tumor suppressor opium-addicted individuals considered confirmatory evidence carcinogenesis opium. Further studies required figure out role alterations evolution among